Optimizing the Mushroom Yield | Reishi


If you want to increase the mushroom yield, then having the right substrate mixture is an important factor.

In today’s video, I go, therefore, over three research papers that tested different substrate types and supplementation to increase the mushroom yield of Ganoderma lucidum, known as Reishi.

The first study tested 6 different strains and used a mixture of wood chips and sawdust from the following trees. Poplar, spruce, pine, larix, and birch. For birch the authors used only wood chips.

The substrate was moistened until 65 to 75% and autoclaved at 125°C for 45 minutes. After the cool down, the substrates were inoculated and incubated at 22°C/72°F in the dark.

The lowest yield could be found on Larix, the highest on birch, followed by poplar. While the highest number of fruiting bodies were found on poplar, followed by birch and spruce.

In the second study, the authors used a mixture of poplar sawdust and wheat bran, supplemented with gluten or molasses at different levels.

The mixtures were autoclaved at 121°C for 1.5 hours.

The highest yield was found using 1% molasses, followed by 1% gluten. Increasing the percentage of molasses or gluten decreased the yield.

These values then translate into the following biological efficiencies. 20.4 for 1% molasses and 19 for 1% gluten.

The third study investigated the following sawdust types. Mahogany, keruing, teak, beechwood, and champaca.

The sawdust was supplemented either with rice bran or wheat bran as well as calcium carbonate. The mixtures were autoclaved at 120°C no duration was given.

The results were quite interesting. Reishi produced mushrooms only on mahogany and keruing. With the highest yield found on mahogany, which was supplemented with wheat bran.

But the biological efficiency using Mahogany was only 7.6. Supplementing with rice bran reduced the yield by 43%.

What can we learn from these three studies for the cultivation of Reishi?

  1. Choosing the right sawdust type is crucial.
  2. Using the right supplement is critical.
  3. Adding different carbon sources can improve the mushroom yield.
  4. Sterilization of sawdust is usually done between 120°C and 125°C for 90 minutes or 45 minutes, respectively.

Talk to you in the next video.


E.Iclal Erkel. Kocaeli University, Arslanbey Agricultural Vocational School, Kocaeli-Turkey. Article Number – E8B8F6738193 Vol.4(11), pp. 1331-1333 , November 2009 https://doi.org/10.5897/AJAR.9000768

Cortina-Escribano, M.; Pihlava, J.-M.; Miina, J.; Veteli, P.; Linnakoski, R.; Vanhanen, H. Effect of Strain, Wood Substrate and Cold Treatment on the Yield and β-Glucan Content of Ganoderma lucidum Fruiting Bodies. Molecules 2020, 25, 4732. https://doi.org/10.3390/molecules25204732

Subarna Roy, Miskat Ara Akhter Jahan, Kamal Kanta Das, Saurab Kishore Munshi, Rashed Noor, Artificial Cultivation of Ganoderma lucidum (Reishi Medicinal Mushroom) Using Different Sawdusts as Substrates, American Journal of BioScience. Vol. 3, No. 5, 2015, pp. 178-182. doi: 10.11648/j.ajbio.20150305.13