Optimum Growing Conditions for the Mycelium of Lion’s Mane


Growing high-quality mushrooms depends on finding the best mushroom strain. It is, therefore, necessary to run experiments to analyze the vegetative growth of several strains.

Today’s research paper addressed this topic and investigated different growing conditions of four strains of Hericium erinaceus, better known as the Lion’s Mane.

All four strains were grown on potato dextrose agar (PDA) and incubated at 25°C/77°F in the dark.

The authors then tested the growth for different temperatures and pH values. They found that the highest growth was observed at 25°C/77°F. Except for one strain which had a similar growth at 20°C/68°F

When it comes to the pH value, the authors found the highest growth at a pH value of 6.

They then analyzed the four strains on 10 different agar media and found the highest growth on potato dextrose agar, Hennerberg, yeast malt extract, and glucose peptone.

Which then lead to the investigation of 10 different carbon sources and 10 different nitrogen sources. The authors found that as carbon sources, dextrose, fructose, and glucose lead to the highest growth. While for the nitrogen sources, the highest growth was found using alanine and ammonium acetate.

The final test was to grow these four strains on three different ratios of sawdust and rice bran. The highest growth was found on a mixture of 90% sawdust and 10% rice bran. Followed by 80% to 20%.

With the information at hand, it should be possible for you to improve the mycelial growth of your lion’s mane strain.

Talk to you in the next video.


📝Imtiaj A, Jayasinghe C, Lee GW, et al. Vegetative Growth of Four Strains of Hericium erinaceus Collected from Different Habitats. Mycobiology. 2008;36(2):88-92. doi:10.4489/MYCO.2008.36.2.088


Finding the right culture media can be tedious. I, therefore, put to getter an eBook containing over 70 different culture media and matched them with tested mushroom strains. You can find the eBook here.

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