Trichoderma is one of the arch-nemesis of every mushroom farmer. If you want to prevent it in the first place, you need to study it. I, therefore, analyzed a research paper in which the authors optimized the cultivation of Trichoderma.
In the paper, the authors studied the effects of carbon, nitrogen, and inorganic salt on the sporulation quantity of Trichoderma. As Trichoderma is used as a biocontrol agent in the agricultural industry, this analysis aimed to obtain the highest biomass of Trichoderma, It promotes the growth of crops and reduces pesticides, thereby improving soil quality and reducing environmental pollution.
For the study, the authors added different carbon sources to the culture medium. They incubated it at 28°C and 180 rpm for five days, and the dry weight of Trichoderma was measured. The highest biomass could be found using maltose, followed by sucrose and glucose. The lowest yield was found using lactose.
To test the effect of different nitrogen sources, the authors added either potassium nitrate, urea, ammonium sulfate, beef cream, peptone, or yeast extract to the culture medium. The medium was then incubated at 28°C and 180 rpm for five days, after which the dry weight of Trichoderma was measured. The best result could be achieved using peptone, followed by yeast extract and beef cream. Using urea led to the lowest biomass.
To test the effect of inorganic salt ions, the authors added either magnesium sulfate, ferrous sulfide, potassium chloride, calcium chloride, sodium chloride, or manganese sulfate to the culture medium. They incubated it at 28°C and 180 rpm for five days, after which the dry weight of Trichoderma was measured. Using magnesium sulfate led to the highest biomass, followed by calcium chloride and sodium chloride. Adding manganese sulfate led to the lowest biomass.
If you want to know how to avoid Trichoderma, then the video on the right is for you. In this video, I put together almost everything you need to know.
Talk to you there.
📝Sui Ming et al 2019 IOP Conf. Ser.: Mater. Sci. Eng. 612 022075, doi:10.1088/1757-899X/612/2/022075, http://creativecommons.org/licenses/by/3.0  Sood, M.; Kapoor, D.; Kumar, V.; Sheteiwy, M.S.; Ramakrishnan, M.; Landi, M.; Araniti, F.; Sharma, A. Trichoderma: The “Secrets” of a Multitalented Biocontrol Agent. Plants 2020, 9, 762. https://doi.org/10.3390/plants9060762, https://creativecommons.org/licenses/by/4.0/