Transcript

As mushrooms are more and more recognized for their potential as a functional food, I will focus in today’s video on the cultivation of Phellinus linteus, known as the black hoof mushroom.

Let’s get started.

To find the best culture media for cultivation, the author tested the first four types. The highest growth was found on MCM, followed by MEA, YMA, and PDA with an optimal pH value of 6 to 7 and a temperature range between 25°C and 30°C (77 to 86°F). 

As the carbon source, as well as the nitrogen source, play an essential role when it comes to mycelial growth and mycelial density, the authors tested several types of them. For the carbon sources, they found the highest growth on sucrose, mannose, and glucose. Using these three carbon sources led to a compact mycelial density.

Analyzing the nitrogen sources, they found the highest growth using potassium nitrate, sodium nitrate, and ammonium tartrate. Using both potassium nitrate and sodium nitrate lead to a compact mycelial density, while for ammonium tartrate, the mycelial density was only somewhat compact.

After finding the optimal culture media and cultivation parameter, the authors tested different types of wood. They analyzed the carbon to nitrogen ratio reduction to find the lowest reduction possible.

They found that using Alnus and Populus led to the highest c/n ratio reduction while using Morus to the lowest, which the authors then used for the cultivation of the black hoof mushroom.

Besides the wood type, the log size seems to impact the colonization rate as the rate of colonization tends to increase with shorter log length.

When it comes to sterilization, the authors found that the highest growth rate was found after autoclaving of the logs for 14 hours at 121°C (250°F). 

After checking the moisture content, the authors found that moisture content of 40% led to a mycelial rate of 25 to 27%, with a general tendency of a high growth rate with higher moisture content.

To test the effect of spawn inoculation methods, the authors compared drilling, log-end sandwich, and sterilized short logs with each other. Here, using the sterilized short log method to the highest initial colonization rate compared to the two others.

Side note: The logs used to test the drilling and log-end sandwich method did not get sterilized.

But, to see the effect of the incubation, the logs had to be buried for 5 months or longer.

An important part when it comes to pinhead formation is the burial depth of the logs. The authors found that the best formation of the pinheads was found on the logs, which were buried one-quarter or one-half their length as it has a direct influence on the temperature and humidity.

The authors found that 31 to 35°C (88 to 95°F) and a relative humidity of 96% or greater led to a good formation of the fruitbodies, which were formed after 1 year after the inoculation. 

Talk to you in the next video.

Source

📝Hur H. Cultural Characteristics and Log-Mediated Cultivation of the Medicinal Mushroom, Phellinus linteus. Mycobiology. 2008;36(2):81-87. doi:10.4489/MYCO.2008.36.2.081